Protein Biochemist - Research Scientist

Position Purpose

The Dartmouth Institute for Biomolecular Targeting (bioMT) is seeking an experienced staff member to join its Core Facilities (Molecular Tools Core, and Molecular Interactions and Imaging Core). The successful applicant will possess knowledge and hands-on experience on the design, production, and biophysical characterization of recombinant proteins. The Protein Biochemist will employ high-throughput approaches for construct design and protein expression and develop purification workflows involving multiple chromatographic methods (e.g., AP, IEC, SEC). Previous experience with structural analysis (x-ray, or cryoEM) of proteins and/or in proteomics will be highly desirable. This person will actively contribute to and facilitate multidisciplinary research projects across the Dartmouth community. Other activities include but are not limited to, data collection and reporting, equipment maintenance, and training of bioMT investigators.

Key Accountabilities

Research/Experiments (65%)

• Works collaboratively with core facility staff, faculty, scientists, and graduate students from across the Dartmouth community to express and purify recombinant proteins for use in research projects.
• Independently designs optimal constructs for protein production in E. coli, HEK293 suspension cell lines, and Sf9/Hi5 cells.
• Independently optimizes expression conditions and develops purification protocols for diverse protein targets.
• Develops proficiency in laboratory equipment for protein expression, purification, and characterization, including AKTA systems, centrifuges, and a variety of analytical instruments.
• Gains experience with diverse biochemical and biophysical techniques, including SPR, ITC, Fluorescence polarization.
• Supports proteomics projects by helping with sample preparation, LC-MS/MS applications, and data analysis.
• Prepare and optimize protein samples for structure determination by X-ray crystallography or cryoEM to support structural biology research projects.
• As needed, supports eukaryotic cell culture services, including culture maintenance and transfection.
• Troubleshoots and recommends solutions to problems associated with experimental design, procedures, and equipment.

Data Collection/Preparation (15%)

• Collects, analyzes, and interprets data resulting from expression and purification experiments.
• Performs literature searches to develop information of relevance to current projects.
• Prepares appropriate and understandable representations of data such as sequence files, graphs, charts, etc.
• Participates in the preparation of annual reports.
• Presents data at laboratory meetings, departmental retreats, and scientific conferences.
• Contributes to the preparation of scientific manuscripts by drafting portions of the text, preparing graphs, tables, and charts, etc. as needed.

Laboratory (10%)

• Adheres to laboratory safety requirements including general, biological, and chemical/hazardous waste safety disposal methods.
• Develops SOPs and other documentation for common procedures and methods.
• Develops and tracks quality-assurance and quality-control metrics.
• Maintains hardcopy and computerized records associated with laboratory activities.

Training (10%)

• Provides training in laboratory methods, procedures, and techniques to multiple levels of scientific investigators (graduate students, post-doctoral fellows, staff scientists and faculty).
• Develops instructional materials as needed.
• Tutors in week-long workshops for undergraduates and graduate students (~3x/year).

Communication

• Communicates and coordinates closely with co-directors and core staff, including staff of the Molecular Imaging and Interactions Core (MIIC) within bioMT.
• Maintains productive collaborations with bioMT investigators.
• Cultivates a diverse, inclusive, and respectful environment.

Performs other duties as assigned.

Required

Education:

• PhD or equivalent experience in biochemistry, molecular biology, bioengineering, or related field is required.

Experience:

• Four years of relevant research experience or the equivalent working in a laboratory is highly desirable.

Skills & Knowledge

• High competency in basic laboratory skills (pipetting, weighing, sterile technique, and recordkeeping), molecular cloning, and one or more of bacterial, insect, or mammalian cell culture.
• Experience with protein purification methods and equipment; Previous experience with structural biology is a plus.
• Ability to learn, develop, and apply new techniques.
• Ability to work independently and apply critical thinking and sound judgment.
• Good organizational and time management skills; Ability to multitask
• Ability to draft clear technical protocols.
• Willingness to work with hazardous materials including biological agents and/or hazardous chemicals.
• Knowledge of computers and software applications.
• Excellent communication skills.
• Willingness to work closely with diverse groups of students and research staff, including the ability to manage and resolve conflicts.

Please submit a CV, Coverletter, and 3 references for review.