New

Research Technician I

Massachusetts General Hospital
United States, Massachusetts, Boston
185 Cambridge Street (Show on map)
May 11, 2026
The Stanton Laboratory in the Center for Genomic Medicine (CGM) at Massachusetts General Hospital (MGH) is seeking a motivated, enthusiastic candidate to join our research group. Our research group works with brain 3D organoid model to understand the biological complexity, target identification, and validation in neurodegenerative disease. We are seeking a highly motivated Research Technician to join our research team focused on human induced pluripotent stem-cell (iPSC) models, gene editing, cellular phenotyping, and 3D brain organoid systems. The successful candidate will play a central role in supporting projects involving iPSC culture, CRISPR-based genome editing, directed differentiation, molecular cloning, and downstream validation assays. This position is ideal for someone with strong hands-on experience in mammalian cell culture and molecular biology who is excited to contribute to a fast-paced, collaborative research environment. The technician will be expected to maintain rigorous experimental records, generate reproducible data, and assist in day-to-day laboratory operations. Does this position require Patient Care? No oPerform laboratory experiments following established protocols and procedures. oPrepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines. oOperate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed. oRecord experimental data accurately and maintain organized records of procedures, protocols, and results. oCompile and analyze data using appropriate statistical and analytical methods. oAssist in data interpretation and presentation, contributing to research ndings and reports. Key responsibilities: Maintain and expand human iPSC lines under sterile culture conditions Conduct CRISPR-based gene editing workflows in iPSCs, including transfection/nucleofection, lentiviral transduction, clone handling, and expansion Support generation, validation, and maintenance of edited cell lines Perform directed differentiation of iPSCs into relevant downstream cell types Generate, culture, and maintain 3D brain organoids Assist with optimization and routine handling of organoid-based workflows Validate differentiated cells and organoid-derived cultures using - Flow cytometry, Immunofluorescence staining and imaging, and perform functional assay as required Carry out molecular cloning tasks such as plasmid preparation, bacterial transformation, restriction digest analysis, Gibson assembly, PCR, and construct verification Assist with genotyping, sequencing library preparation, and clone validation Maintain accurate, organized, and detailed lab notebooks and experimental records Contribute to protocol optimization, troubleshooting, and experimental planning Present data clearly in lab meetings and communicate progress to the research team Prior experience below is a plus: Prior experience with human iPSC culture Experience with CRISPR/Cas9 gene editing Experience with stem cell differentiation Experience generating or maintaining brain organoids or other 3D culture systems Familiarity with aseptic technique, nucleofection, lentiviral production, and sequencing-based validation Previous experience in an academic, biotech, or translational research laboratory Education Bachelor's Degree Related Field of Study required Can this role accept experience in lieu of a degree? Yes Licenses and Credentials Experience Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred Knowledge, Skills and Abilities - Strong theoretical and practical knowledge of laboratory techniques and procedures. - Familiarity with common laboratory equipment and instruments used in research experiments. - Proficiency in data collection, analysis, and scientific software tools. - Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively. - Strong problem-solving skills and the ability to troubleshoot technical issues. - Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges. The General Hospital Corporation is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.